English: igure 5. Localization of filaggrin-2 in human epidermis.

(A) Immunohistochemical analyses of FLG2 in human skin sections. Healthy skin sections from palmar (panel a; scale bar, 60 µm) and plantar (panel c; scale bar, 120 µm) sites as well as the upper leg region (panel b; scale bar, 60 µm) were stained with anti-FLG2 antibody. Specificity of antibody was confirmed by blocking the antibody with the antigen (panel d; scale bar, 60 µm). Representative experiments out of three are shown. (B) Post-embedding immunoelectron microscopy of FLG2 (10 nm gold) in healthy foreskin. FLG2 labels are present in both granular and cornified cells (panels a and b). Note that FLG2 labels are either diffusely dispersed in the cytoplasm (panel c) or grouped as keratohyalin granules (panel d). SG, stratum granulosum; SC, stratum corneum.

doi:10.1371/journal.pone.0005227.g005